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Journal of Animal Science and Technology
Vol. 56, 57(2014~)
▣ 편집위원장 : 최인호
▣ 편집위원 : 정호영, 최창원, 이철영, 오세종, 김관석,
    이홍구, 송민호, 김은중, 조철훈

논문번호 :
학회지년월 : 201807
접수일자 : 2018-02-01 채택일자 : 2018-07-13
전공분야 : 번식생리및내분비
조 회 수 : 2
Examination of the xanthosine response on
gene expression of mammary epithelial
cells using RNA-seq technology

Shanti Choudhary1, Wenli Li2, Derek Bickhart2, Ramneek Verma1, R. S. Sethi1, C. S. Mukhopadhyay1
and Ratan K. Choudhary1*

Background: Xanthosine treatment has been previously reported to increase mammary stem cell population and
milk production in cattle and goats. However, the underlying molecular mechanisms associated with the increase in
stem cell population and milk production remain unclear.
Methods: Primiparous Beetal goats were assigned to the study. Five days post-partum, one mammary gland of
each goat was infused with xanthosine (TRT) twice daily (2×) for 3 days consecutively, and the other gland served
as a control (CON). Milk samples from the TRT and CON glands were collected on the 10th day after the last
xanthosine infusion and the total RNA was isolated from milk fat globules (MEGs). Total RNA in MFGs was mainly
derived from the milk epithelial cells (MECs) as evidenced by expression of milk synthesis genes. Significant
differentially expressed genes (DEGs) were subjected to Gene Ontology (GO) terms using PANTHER and gene
networks were generated using STRING db.
Results: Preliminary analysis indicated that each individual goat responded to xanthosine treatment differently,
with this trend being correlated with specific DEGs within the same animal’s mammary gland. Several pathways
are impacted by these DEGs, including cell communication, cell proliferation and anti-microbials.
Conclusions: This study provides valuable insights into transcriptomic changes in milk producing epithelial cells in
response to xanthosine treatment. Further characterization of DEGs identified in this study is likely to delineate the
molecular mechanisms of increased milk production and stem or progenitor cell population by the xanthosine
Keywords: Goat, Milk fat globule, RNA sequencing, Xanthosine, RT-qPCR
논문번호 :
학회지년월 : 201806
접수일자 : 2017-10-31 채택일자 : 2018-06-22
전공분야 : 단위가축영양
조 회 수 : 6
Correction to: Physicochemical properties of M. longissimus dorsi of Korean native pigs

Gye-Woong Kim and Hack-Youn Kim

논문번호 :
학회지년월 : 201806
접수일자 : 2018-01-31 채택일자 : 2018-06-11
전공분야 : 번식생리및내분비
조 회 수 : 12
Analysis of protein-protein interaction
network based on transcriptome profiling
of ovine granulosa cells identifies candidate
genes in cyclic recruitment of ovarian

Reza Talebi1, Ahmad Ahmadi1* and Fazlollah Afraz2

After pubertal, cohort of small antral follicles enters to gonadotrophin-sensitive development, called recruited follicles.
This study was aimed to identify candidate genes in follicular cyclic recruitment via analysis of protein-protein interaction
(PPI) network. Differentially expressed genes (DEGs) in ovine granulosa cells of small antral follicles between follicular and
luteal phases were accumulated among gene/protein symbols of the Ensembl annotation. Following directed graphs,
PTPN6 and FYN have the highest indegree and outdegree, respectively. Since, these hubs being up-regulated in ovine
granulosa cells of small antral follicles during the follicular phase, it represents an accumulation of blood immune cells
in follicular phase in comparison with luteal phase. By contrast, the up-regulated hubs in the luteal phase including CDK1,
INSRR and TOP2A which stimulated DNA replication and proliferation of granulosa cells, they known as candidate genes
of the cyclic recruitment.
Keywords: Protein–protein interaction network, Biomarkers, Ovarian follicles, Granulosa cells, Cyclic recruitment
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