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Journal of Animal Science and Technology
Vol. 56, 57(2014~)
▣ 편집위원장 : 최인호
▣ 편집위원 : 정호영, 최창원, 이철영, 오세종, 김관석,
    이홍구, 송민호, 김은중, 조철훈

논문번호 :
학회지년월 : 201807
접수일자 : 2018-02-01 채택일자 : 2018-07-13
전공분야 : 번식생리및내분비
조 회 수 : 8
Examination of the xanthosine response on
gene expression of mammary epithelial
cells using RNA-seq technology

Shanti Choudhary1, Wenli Li2, Derek Bickhart2, Ramneek Verma1, R. S. Sethi1, C. S. Mukhopadhyay1
and Ratan K. Choudhary1*

Background: Xanthosine treatment has been previously reported to increase mammary stem cell population and
milk production in cattle and goats. However, the underlying molecular mechanisms associated with the increase in
stem cell population and milk production remain unclear.
Methods: Primiparous Beetal goats were assigned to the study. Five days post-partum, one mammary gland of
each goat was infused with xanthosine (TRT) twice daily (2×) for 3 days consecutively, and the other gland served
as a control (CON). Milk samples from the TRT and CON glands were collected on the 10th day after the last
xanthosine infusion and the total RNA was isolated from milk fat globules (MEGs). Total RNA in MFGs was mainly
derived from the milk epithelial cells (MECs) as evidenced by expression of milk synthesis genes. Significant
differentially expressed genes (DEGs) were subjected to Gene Ontology (GO) terms using PANTHER and gene
networks were generated using STRING db.
Results: Preliminary analysis indicated that each individual goat responded to xanthosine treatment differently,
with this trend being correlated with specific DEGs within the same animal’s mammary gland. Several pathways
are impacted by these DEGs, including cell communication, cell proliferation and anti-microbials.
Conclusions: This study provides valuable insights into transcriptomic changes in milk producing epithelial cells in
response to xanthosine treatment. Further characterization of DEGs identified in this study is likely to delineate the
molecular mechanisms of increased milk production and stem or progenitor cell population by the xanthosine
Keywords: Goat, Milk fat globule, RNA sequencing, Xanthosine, RT-qPCR
논문번호 :
학회지년월 : 201807
접수일자 : 2018-03-29 채택일자 : 2018-07-26
전공분야 : 축산물및가공
조 회 수 : 7
Changes in free amino acid content and
hardness of beef while dry-aging with
Mucor flavus

Takashi Hanagasaki1,3* and Naokazu Asato2,4

Background: A mold strain thought to be suitable for dry-aging process was isolated. The information about the
scientific aspects of molds related to dry-aging beef is scarce. We, therefore, conducted aging trials to determine the
characteristics of the isolated mold strain associated with dry-aging process. Specifically, during the dry-aging of beef
with the mold strain, the changes in the free amino acid content, hardness, productive loss, drip and cooking loss were
analyzed. These characteristics were compared with those obtained while dry-aging in the absence of a mold.
Results: The isolated mold strain was identified as Mucor flavus. The free amino acid content in the mold-aging beef
decreased or remained constant during the aging process. However, that in the trimming sections of the beef
dramatically increased in the presence of mold. In addition, hardness of mold-aging beef gradually decreased during
the aging process and finally decreased significantly.
Conclusion: Amino acids such as GABA (gamma-aminobutyric acid), proline, and aspartic acid were produced by our
mold strain, M. flavus during its growth on beef meat, and the mold conferred savory odors to the dry-aged beef
논문번호 :
학회지년월 : 201806
접수일자 : 2017-10-31 채택일자 : 2018-06-22
전공분야 : 단위가축영양
조 회 수 : 7
Correction to: Physicochemical properties of M. longissimus dorsi of Korean native pigs

Gye-Woong Kim and Hack-Youn Kim

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