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Journal of Animal Science and Technology
Vol. 56, 57(2014~)
▣ 편집위원장 : 최인호
▣ 편집위원 : 정호영, 최창원, 이철영, 오세종, 김관석,
    이홍구, 송민호, 김은중, 조철훈

논문번호 :
학회지년월 : 201710
접수일자 : 2017-05-02 채택일자 : 2017-10-09
전공분야 : 번식생리및내분비
조 회 수 : 8
The use of infrared thermography to detect the stages of estrus cycle and ovulation time in anatolian shepherd dogs

Kemal Tuna Olğaç1*, Ergun Akçay1, Beste Çil1, Burak Mehmet Uçar2 and Ali Daşkın1

Background: The aim of the study is to evaluate the effectiveness of thermographic monitoring, using the temperature changes of perianal and perivulvar areas for the determination of estrus in Anatolian Shepherd bitches. Fifteen bitches were used in the study. Blood and vaginal smear samples were collected and thermographic monitoring of perianal and perivulvar areas were carried out starting from proestrus to early diestrus. Also, external signs of estrus were investigated. Smear samples were evaluated by light microscopy after Diff-Quik staining method and superficial and keratinized superficial cells were determined as percentage (S + KS%). Progesterone and luteinizing hormone measurements were done by radioimmunoassay. The difference in temperature between perianal and perivulvar areas was evaluated through thermographic images by FLIR ResearchIR Software. Results: According to the results obtained from the study, differences between progesterone and S + KS% were statistically significant (P < 0,05). Although temperature showed increase and decrease with progesterone and S + KS%, the differences were not important statistically (P > 0,05). Serum luteinizing hormone levels did not sign any difference (P > 0,05). Conclusions: As a result, thermographic monitoring alone is not enough for estrus detection in Anatolian Shepherd bitches. However, it can be used to assist the actual estrus detection technique in terms of providing some foreknowledge by evaluating the differences in temperature. Keywords: Bitch, Estrus, Estrus detection, Ovulation, Thermography
논문번호 :
학회지년월 : 201710
접수일자 : 2017-04-24 채택일자 : 2017-10-16
전공분야 : 단위가축영양
조 회 수 : 5
Apparent and standardized ileal nutrient
digestibility of broiler diets containing
varying levels of raw full-fat soybean
and microbial protease

Mammo M. Erdaw1,3*, Rider A. Perez-Maldonado2 and Paul A. Iji1*

Background: Although soybean meal (SBM) is excellent source of protein in diets for poultry, it is sometimes
inaccessible, costly and fluctuates in supply. The SBM can partially be replaced by full-fat SBM, but the meals
prepared from raw full-fat soybean contain antinutritional factors. To avoid the risk of antinutritional factors, heat
treatment is always advisable, but either excessive or under heating the soybean could negatively affect the quality.
However, the potential for further improvement of SBM by supplementing with microbial enzymes has been
suggested by many researchers. The objective of this study was to evaluate the performance and ileal nutrient
digestibility of birds fed on diets containing raw soybeans and supplemented with microbial protease.
Methods: A 3 × 2 factorial, involving 3 levels of raw full-fat soybean (RFFS; 0, 45 or 75 g/kg of diet) and 2 levels of
protease (0 or 15,000 PROT/kg) was used. The birds were raised in a climate-controlled room. A nitrogen-free diet
was also offered to a reference group from day 19 to 24 to determine protein and amino acid flow at the terminal
ileum and calculate the standardized ileal digestibility of nutrients. On days 10, 24 and 35, body weight and feed
leftover were recorded to calculate the body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR).
On day 24, samples of ileal digesta were collected at least from two birds per replicate.
Results: When RFFS was increased from 0 to 75 g/kg of diet, the content of trypsin inhibitors was increased from
1747 to 10,193 trypsin inhibitors unit (TIU)/g of diets, and feed consumption of birds was also reduced (P < 0.05).
Increasing RFFS level reduced the BWG from hatch 0 to 10 d (P < 0.01) and hatch to 24 d (P < 0.05). The BWG of
birds from hatch to 35 was not significantly (P = 0.07) affected.
Feed intake was also reduced (P < 0.05) during 0 to 35 d. However, protease supplementation improved (P < 0.05)
the BWG and FCR during 0 to 24 d. Rising levels of RFFS increased the weight of pancreas (P < 0.001) and small
intestine (P < 0.001) at day 24. Except for methionine, apparent and the corresponding standardized ileal
digestibility of CP and AA were reduced (P < 0.01) by increasing levels of RFFS in diets.
Conclusion: This study showed that some commercial SBM could be replaced by RFFS in broiler diets, without
markedly compromising productivity. The AID and SID of CP and lysine were slightly improved by dietary
supplementation of microbial protease.
Keywords: Amino acids, Antinutritional factors, Broilers, Ileal digestibility, Microbial protease, Trypsin inhibitors
논문번호 :
학회지년월 : 201709
접수일자 : 2017-03-08 채택일자 : 2017-09-25
전공분야 : 기타
조 회 수 : 8
A study of ribonuclease activity in venom
of vietnam cobra

Thiet Van Nguyen1* and A. V. Osipov2

Background: Ribonuclease (RNase) is one of the few toxic proteins that are present constantly in snake venoms of
all types. However, to date this RNase is still poorly studied in comparison not only with other toxic proteins of snake
venom, but also with the enzymes of RNase group. The objective of this paper was to investigate some properties of
RNase from venom of Vietnam cobra Naja atra.
Methods: Kinetic methods and gel filtration chromatography were used to investigate RNase from venom of Vietnam
Results: RNase from venom of Vietnam cobra Naja atra has some characteristic properties. This RNase is a thermostable
enzyme and has high conformational stability. This is the only acidic enzyme of the RNase A superfamily exhibiting a high
catalytic activity in the pH range of 1&#8211;4, with pHopt = 2.58 ± 0.35. Its activity is considerably reduced with increasing ionic
strength of reaction mixture. Venom proteins are separated by gel filtration into four peaks with ribonucleolytic activity,
which is abnormally distributed among the isoforms: only a small part of the RNase activity is present in fractions of
proteins with molecular weights of 12&#8211;15 kDa and more than 30 kDa, but most of the enzyme activity is detected in
fractions of polypeptides, having molecular weights of less than 9 kDa, that is unexpected.
Conclusions: RNase from the venom of Vietnam cobra is a unique member of RNase A superfamily according to its
acidic optimum pH (pHopt = 2.58 ± 0.35) and extremely low molecular weights of its major isoforms (approximately
8.95 kDa for RNase III and 5.93 kDa for RNase IV).
Keywords: Acidic optimum pH, Conformational stability, Gel filtration, Ionic strength, Isoforms, Thermostability, Venom
RNase, Vietnam cobra
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